Coding

Part:BBa_K3046028:Design

Designed by: Philip Srensen   Group: iGEM19_DTU-Denmark   (2019-07-04)


PyrG CDS from Aspergillus fumigatus


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 434
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 434
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 172
    Illegal XhoI site found at 32
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 434
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 434
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 225


Design Notes

This CDS encodes the PyrG gene which can be used as an auxotrophic marker.

Source

Sequence originates from Aspergillus flavus.

References